Enzymatische Umwandlungen von Steroiden. I. Mitteilung. Vergleich der Steroidumwandlung in Leber und Nebenniere

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Abstract
Rat liver and bovine adrenal homogenates show the following similarities and dissimilarities in the transformations of various steroid groups of functions. Both liver and adrenal homogenates can reduce the C11 keto to C11 hydroxy and introduce a hydroxy group at the C11 position. Neither liver nor adrenal homogenates exhibit oxidation of a-keto steroids on C17. Neither homogenate can remove the side chain of cholesterol or cholestenon. Adrenal homogenates oxidize 3-hydroxy-5-precursors to 3-keto-4 steroids while the latter compound is reduced by liver homogenates. Only the adrenal homogenates introduce oxygen functions on C17 and C21. Liver homogenates cause reductive metabolism of adrenal cortical steroids while adrenal homogenates cause oxidative metabolism. The yields of transformation products was not altered by the addition of ACTH. A method for the qualitative and quantitative determination of adrenal cortical hormones in tissue homogenates is outlined.