Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles
- 10 December 1999
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 2 (1) , 42-49
- https://doi.org/10.1038/71366
Abstract
Here, to study lipid–protein interactions that contribute to the biogenesis of regulated secretory vesicles, we have developed new approaches by which to label proteins in vivo, using photoactivatable cholesterol and glycerophospholipids. We identify synaptophysin as a major specifically cholesterol-binding protein in PC12 cells and brain synaptic vesicles. Limited cholesterol depletion, which has little effect on total endocytic activity, blocks the biogenesis of synaptic-like microvesicles (SLMVs) from the plasma membrane. We propose that specific interactions between cholesterol and SLMV membrane proteins, such as synaptophysin, contribute to both the segregation of SLMV membrane constituents from plasma-membrane constituents, and the induction of synaptic-vesicle curvature.Keywords
This publication has 49 references indexed in Scilit:
- Linking cargo to vesicle formation: receptor tail interactions with coat proteinsPublished by Elsevier ,2002
- FUNCTIONS OF LIPID RAFTS IN BIOLOGICAL MEMBRANESAnnual Review of Cell and Developmental Biology, 1998
- Protein and lipid sorting from thetrans–Golgi network to secretory granules—recent developmentsSeminars in Cell & Developmental Biology, 1998
- Protein and lipid sorting between the endoplasmic reticulum and the Golgi complexSeminars in Cell & Developmental Biology, 1998
- Functional rafts in cell membranesNature, 1997
- Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surfacePublished by Elsevier ,1992
- Identification of the Lipid-Binding Site of Phosphatidylcholine-Transfer Protein with Phosphatidylcholine Analogs Containing Photactivable Carbene PrecursorsEuropean Journal of Biochemistry, 1983
- Syntheses of Phosphatidylcholines, Sphingomyelins and Cholesterol Substituted with Azido Fatty Acids. Photocrosslinking with Nearest Neighbouring Lipids in Liposomes Chemical and Mass Spectroscopic ProofHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1982
- Phospholipids Containing Photoactivable Groups in Studies of Biological Membranes *Annals of the New York Academy of Sciences, 1980
- Covalent Cross-linking of Photosensitive Phospholipids to Human Serum High Density Apolipoproteins (apoHDL)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1979