Increased throughput in quantitative bioanalysis using parallel‐column liquid chromatography with mass spectrometric detection

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Abstract
The feasibility of quantitative bioanalysis by parallel‐column liquid chromatography in conjunction with a conventional single‐source electrospray mass spectrometer has been investigated using plasma samples containing a drug and its three metabolites. Within a single chromatographic run time, sample injections were made alternately onto each of two analytical columns in parallel at specified intervals, with a mass spectrometer data file opened at every injection. Thus, the mass spectrometer collected data from two sample injections into separate data files within a single chromatographic run time. Therefore, without sacrificing the chromatographic separation or the selected reaction monitoring (SRM) dwell time, the sample throughput was increased by a factor of two. Comparing the method validation results obtained using the two‐column system with those obtained using the corresponding conventional single‐column approach, the methods on the two systems were found to be equivalent in terms of accuracy and precision. The parallel‐column system is simple and can be implemented using existing laboratory equipment with no additional capital outlays. A parallel‐column system configured in this manner can be used not only for the within‐a‐run analysis of two samples containing two different sets of chemical entities, but also for the within‐a‐run analysis of two samples containing the same set of chemical entities. Copyright © 2001 John Wiley & Sons, Ltd.

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