Far-Field Optical Nanoscopy
Top Cited Papers
- 25 May 2007
- journal article
- review article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 316 (5828) , 1153-1158
- https://doi.org/10.1126/science.1137395
Abstract
In 1873, Ernst Abbe discovered what was to become a well-known paradigm: the inability of a lens-based optical microscope to discern details that are closer together than half of the wavelength of light. However, for its most popular imaging mode, fluorescence microscopy, the diffraction barrier is crumbling. Here, I discuss the physical concepts that have pushed fluorescence microscopy to the nanoscale, once the prerogative of electron and scanning probe microscopes. Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.Keywords
This publication has 53 references indexed in Scilit:
- Wide-field subdiffraction imaging by accumulated binding of diffusing probesProceedings of the National Academy of Sciences, 2006
- H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopyProceedings of the National Academy of Sciences, 2006
- Single-molecule mountains yield nanoscale cell imagesNature Methods, 2006
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Nature Methods, 2006
- Macromolecular-scale resolution in biological fluorescence microscopyProceedings of the National Academy of Sciences, 2006
- Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteinsProceedings of the National Academy of Sciences, 2005
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolutionProceedings of the National Academy of Sciences, 2005
- Imaging and writing at the nanoscale with focused visible light through saturable optical transitionsApplied Physics A, 2003
- Toward fluorescence nanoscopyNature Biotechnology, 2003