Asymmetry of chromatin subunits probed with histone H1 in an H1-DNA complex
- 1 May 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (10) , 1880-1883
- https://doi.org/10.1021/bi00603a012
Abstract
Treatment of nucleosomes [mouse lymphocytic leukemia L-5178Y cells] with a low concentration of sodium dodecyl sulfate removed all proteins except histone H1 from DNA and confirmed previous observation on sheared chromatin. No redistribution of H1 occurred during this procedure for isolation of the H1-DNA complex. The H1-DNA complex was isolated from nucleosome monomer, doubly labeled in its protein and DNA and fractionated according to the length of DNA, and then distribution of H1 was analyzed quantitatively. Apparently the monomer consisted of 2 sspp., one containing 160 base pairs of DNA and 1 molecule of H1, and the other containing 140 base pairs of DNA and no H1. Since no monomer with 2 molecules of H1 was found, the nucleosome core probably has a binding site for H1 on only 1 side and the nucleosome is not a dyad.This publication has 5 references indexed in Scilit:
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