Inactivation of Triphosphopyridine Nucleotide by Ultraviolet Irradiation and the Identification of Decomposition Products

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Abstract
UV irradiation of triphosphopyridine nucleotide (TPN) resulted in the loss of its coenzymatic activity as studied in the glucose-6-phosphate dehydrogenase system. This inactivation is due to the splitting of the molecule. The split products were identified as: (1) nico-tinamide and probably nicotinamide linked to unidentified breakdown products of ribose, (2) adenosine 2'',5''-diphosphate, and (3) a hitherto not described compound identified as 2''-phospho-adenosine diphosphate. The possible transphorylating functions of the latter are being investigated. The adenosine of TPN was recovered quantitatively in the phosphorylated products.