POTASSIUM UPTAKE BY THE DOG ERYTHROCYTE

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Abstract
The inward transport of K by separated dog erythrocytes was studied at concns. of K in the medium from 2.9 to 25.0 m.eq./l. and at 38.0 and 33.0[degree]C. At the physiological concn. of external K (4.06 m. eq./l. medium), the inward K flux is 0.11 m.eq/l. cells/hr. and the glucose consumption is 2.0 m[image]/l. cells/hr. The dependence of K influx on extracellular K concn. is given by the following equation, K influx (m.eq./l. cells hr.) = 0.028 [K] amb. - 0.003 in which [K] amb refers to the K concn. in the medium. In a single 93 hr. expt., 94% of the intracellular K was exchanged at an apparently uniform rate. The avg. apparent activation energy for the process is 7,750 calories 2,000 calories/mol and there is some indication that the apparent activation energy of inward K transport decreases with increasing external K concn.

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