The crystal structure of an RNA oligomer incorporating tandem adenosine- inosine mismatches
Open Access
- 1 October 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (20) , 4117-4122
- https://doi.org/10.1093/nar/25.20.4117
Abstract
The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has been refined to 2.5 Å. It shows a symmetric internal loop of two non-Watson-Crick base pairs which form in the middle of the duplex. The tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both A(anti)-I(anti) mismatches are in a head-to-head conformation forming hydrogen bonds using the Watson-Crick positions. The octamer duplexes stack above one another in the cell forming a pseudo-infinite helix throughout the crystal. A hydrated calcium ion bridges between the 3′-terminal of one molecule and the backbone of another. The tandem A-I mismatches are incorporated with only minor distortion to the backbone. This is in contrast to the large helical perturbations often produced by sheared G-A pairs in RNA oligonucleotides.Keywords
This publication has 28 references indexed in Scilit:
- Solution Structure of (rGGCAGGCC)2 by Two-Dimensional NMR and the Iterative Relaxation Matrix Approach,Biochemistry, 1997
- A curved RNA helix incorporating an internal loop with G·A and A·A non-Watson–Crick base pairingProceedings of the National Academy of Sciences, 1996
- Crystal Structure of a Group I Ribozyme Domain: Principles of RNA PackingScience, 1996
- α Helix-RNA Major Groove Recognition in an HIV-1 Rev Peptide-RRE RNA ComplexScience, 1996
- The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNANature, 1989
- Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairsNucleic Acids Research, 1989
- Inosine.adenine base pairs in a B-DNA duplexNucleic Acids Research, 1987
- Base pairing involving deoxyinosine: implications for probe designNucleic Acids Research, 1985
- Comparative Anatomy of 16-S-like Ribosomal RNAPublished by Elsevier ,1985
- Pairing of inosine with adenosine in codon position two in the translation of polyinosinic acidJournal of Molecular Biology, 1973