Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys

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Abstract
A method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to Lassa, Marburg and Ebola viruses. The technique uses mixtures of specifically infected Vero [African green monkey kidney] cells placed on Teflon-templated microscope slides. It was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly hazadous human pathogens.