Construction of a human chromosome 3 specific Notl linking library using a novel cloning procedure
- 21 November 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (21) , 6319-6324
- https://doi.org/10.1093/nar/18.21.6319
Abstract
Two new diphasmid vectors (lambda SK17 and SK22) and a novel procedure to construct linking libraries are described. A partial filling-in reaction provides counter-selection against false linking clones in the library, and obviates the need for supF selection. The diphasmid vectors, in combination with the novel selection procedure, have been used to construct a chromosome 3 specific Notl linking library from a human chromosome 3/mouse microcell hybrid cell line. The application of the new vectors and the strong biochemical and biological selections resulted in a library of 60.000 Notl linking clones. As practically all of them are real Notl linking clones (no false recombinant8) the library represents approximately 3.000 human recombinants (equal to 10–15 genomic equivalents of chromosome 3). Previously published methods for construction of linking libraries are compared with the procedure described in the present paper. The advantages of the new vectors and the novel protocol are discussed.This publication has 23 references indexed in Scilit:
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