Primary structure of hemoglobin α-chain from Cuckoo (Eudynamys scolopaceae, Cuculiformes)

Abstract
The complete amino acid sequence of the αA-chain of major hemoglobin component from Cuckoo (Eudynamys scolopaceae) is presented. Separation of the polypeptide subunits was achieved by ion exchange chromatography in the presence of 8 M urea. The sequence was studied by automatic Edman degradation of the native chain and its tryptic fragments in a gas-phase sequencer. Comparison with other avian hemoglobins shows residues α21, α30, α96, α110, and α114 as being specific to Cuckoo. The functional significance of these is discussed.

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