Primary structure of hemoglobin α-chain ofColumba livia (gray wild pigeon)

1 October 1989

journal article
research article
Published by Springer Nature in Protein Journal

Vol. 8 (5) , 629-646
https://doi.org/10.1007/bf01025603

Abstract

Primary structure of hemoglobin of α-chain ofColumba livia is presented. The separation of α-chain was obtained from globin by ion-exchange chromatography (CMC-52) and reversed-phase HPLC (RP-2 column). Amino acid sequence of intact as well as tryptic digested chain was determined on gas-phase sequencer. Structure is aligned homologously with 21 other species. Among different exchanges, positions α24 (Tyr→Leu), α26 (Ala→Gly), α32 (Met→Leu), α64 (Asp→Glu), α113 (Leu→Phe), and α129 (Leu→Val) are unique to pigeon hemoglobin. The various exchanges in α-chain are discussed with reference to evolution and phylogeny. The results show that the order Columbiformes is evolutionarily closer to the order Anseriformes. Since the pigeon is homogeneous, having HbA (α^A-chain) and lacks α^D-chain, its phylogenetic placement could be established among birds having single hemoglobin components.

Keywords

This publication has 36 references indexed in Scilit:

Primary structure of the hemoglobin α-chain of rose-ringed parakeet (Psittacula krameri)
Protein Journal, 1988
High-Altitude Respiration of Birds. The Primary Structures of the Major and Minor Hemoglobin Component of Adult Goshawk (Accipiter gentilis,Accipitrinae)
Biological Chemistry Hoppe-Seyler, 1987
High Altitude Respiration of Birds. The Primary Structures of the Major and Minor Hämoglobin Component of Adult European Black Vulture(Aegypius monachus,Aegypiinae)
Biological Chemistry Hoppe-Seyler, 1987
Die Primärstruktur der α- und β-Ketten der Hauptkomponenten der Hämoglobine des Straußes(Struthio camelus)und des Nandus(Rhea americana) (Struthioformes).Aspekte zur Atmungsphysiologie und Systematik
Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
The complete nucleotide sequence of the duck αA-globin gene
Gene, 1982
By-products as an aid in residue identification during peptide sequence analysis with dimethylaminoazobenzene isothiocyanate
Protein Journal, 1982
SHORT COMMUNICATION
Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
Micro‐sequence analysis of peptides and proteins using 4‐NN‐dimethylaminoazobenzene 4′‐isothiocyanate/phenylisothiocyanate double coupling method
FEBS Letters, 1978
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature, 1970
Studies on the structure of hemoglobin I. Physicochemical properties of human globin
Biochimica et Biophysica Acta, 1958