Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
- 1 July 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (13) , 4321-4325
- https://doi.org/10.1073/pnas.82.13.4321
Abstract
The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNA species late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). The mutant''s translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2.alpha. subunit kinase activity. Mutant dl331 can grow with nearly normal kinetics in cells that do not express the kinase.This publication has 45 references indexed in Scilit:
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