Studies of Ligand Binding to Cholera Toxin, III
- 1 January 1978
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 359 (1) , 719-723
- https://doi.org/10.1515/bchm.1978.359.1.719
Abstract
Binding parameters for the interaction of cholera toxin and choleragenoid with monosialo-gangliotetraose, the oligosaccharide moiety of ganglioside II3NeuAc-GgOse4-Cer, were measured by equilibrium displacement dialysis. The experimental data were evaluated by a curve fitting computer program. The binding curves obtained to 6.degree. C reflected positive cooperativity with average Hill coefficients in the range of 1.16-1.25. The cholera toxin and its ganglioside-binding protomer B-protein bind 4 mol of monosialo-gangliotetraitol/mol of protein. The presence of disulfide-cleaving agents like 2-mercaptoethanol did not eliminate the cooperativity of the binding characteristics, but increased the Hill coefficient.This publication has 6 references indexed in Scilit:
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