Quantitative Analysis of Diethyl Ether in Blood

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Abstract
A method for the determination of blood-ether concentration was developed, consisting of refluxlng a sample of blood, condensing the ether in a liquid nitrogen trap and using gas chromatography for analysis. The method is applicable for other volatile anesthetics. A relative standard deviation of [plus or minus]3 per cent was obtained. Recovery of ether added to blood was over 95 per cent. The blood/gas distribution coefficient for ether In human blood at 37[degree]C. was calculated to be 12.