Studies on 3-Indoleacetic Acid Metabolism. III. The Uptake of 3-Indoleacetic Acid by Pea Epicotyls and Its Conversion to 3-Indoleacetylaspartic Acid

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Abstract
Pea epicotyls incubated in indoleacetic acid (IAA) solutions (2.5 to 60 ppm) containing M/60 NaH2PO4 for periods up to 24 hours were analyzed for Salkowski-reactive components by a differential ether extraction technique. IAA and indoleacetylaspartic acid are the predominant components found, their relative amounts depending upon the concentration of IAA applied. Only about 20% of the IAA disappearing from solution could thus be accounted for. If, however, tissues were treated in IAA and placed in a moist chamber, practically all IAA which accumulates in the tissues is converted into indoleacetylaspartic acid. Both the uptake of IAA and its conjugation with aspartic acid are aerobic processes. Implication for IAA-induced growth are discussed.