A Comparison of the Growth Properties of Coxsackie Virus Strains A-9 and A-10 with Poliovirus Strain Mahoney in Cultures of Primary Human Amnion Cells

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Abstract
Summary: Comparable cultures of primary human amnion cells were inoculated with poliovirus strain Mahoney, Coxsackie virus strain A-9 and Coxsackie virus strain A-10TC. The intracellular virus reached a maximal titer between 8 to 12 hr after inoculation for each of the three viruses. The release of virus into the medium followed its production in the cell by about 4 hr, but the maximal amount released by 30 hr after infection did not exceed more than one-fifth of the amount produced (cell associated, intracellular). The cytopathic changes in primary human amnion cells which followed inoculation were identical for each of the three viruses. However, the temporal relationships between the production of these viruses and the appearance of cellular changes were markedly different. The early cytopathic changes preceded slightly the appearance of intracellular poliovirus. Only 30% of the cells were in early stages of cellular change when the maximal intracellular titers of Coxsackie virus A-9 were reached. At the time of the maximal production of Coxsackie A-10TC, less than 10% of the cells were damaged, although 90% eventually underwent characteristic cellular alterations and degeneration. The data for the Coxsackie viruses showed that the cellular changes occurred subsequent to virus formation. The amount of virus produced in individual cells of comparable size was similar for poliovirus strain Mahoney and Coxsackie virus A-10TC, while 4 to 7 times that amount was produced after inoculation with Coxsackie virus A-9.