Quantitative determination of the superoxide radicals in the xanthine oxidase reaction by measurement of the electron spin resonance signal of the superoxide radical spin adduct of 5,5-dimethyl-1-pyrroline-1-oxide.
- 1 January 1984
- journal article
- research article
- Published by Pharmaceutical Society of Japan in Journal of Pharmacobio-Dynamics
- Vol. 7 (8) , 563-569
- https://doi.org/10.1248/bpb1978.7.563
Abstract
Reactivities of biologically active compounds with [carcinogenic] superoxide radicals were examined by measurement of ESR spectra of 5-hydroperoxy-2,2-dimethyl-1-pyrrolidinyloxyl formed from 5,5-dimethyl-1-pyrroline-1-oxide and the superoxide radicals generated by xanthine-xanthine oxidase system. Quantitative determination of the superoxide radicals was performed by comparing the intensity of the spin adducts with that of 4-hydroxy-2,2,6,6-tetramethyl-piperidinooxyl whose spin concentration was determined. Methyl viologen enhanced the formation of this spin adducts, while rutin and luteoskyrin prevented it.This publication has 4 references indexed in Scilit:
- Spin trapping of free radicals during hepatic microsomal lipid peroxidation.Proceedings of the National Academy of Sciences, 1981
- SPIN TRAPPING OF SUPEROXIDE1979
- The Biology of Oxygen RadicalsScience, 1978
- Electron spin resonance-spin trapping. Detection of superoxide formation during aerobic microsomal reduction of nitro-compoundsBiochemical and Biophysical Research Communications, 1978