Phosphodiesterase 4 in osteoblastic osteosarcoma cells as a potential target for growth inhibition
- 1 June 2003
- journal article
- case report
- Published by Wolters Kluwer Health in Anti-Cancer Drugs
- Vol. 14 (5) , 377-381
- https://doi.org/10.1097/00001813-200306000-00009
Abstract
Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that HOSM-1 cells, an osteosarcoma cell line established from human mandible, expressed mRNA for osteoblastic markers, such as alkaline phosphatase, osteonectin, osteocalcin and parathyroid hormone receptor, thus exhibiting an osteoblastic phenotype. We have investigated a possible role of cyclic nucleotide phosphodiesterases (PDEs) in osteosarcoma cells. RT-PCR analysis revealed that HOSM-1 cells expressed mRNA for PDE4A, 4B and 4C. In addition, rolipram, a specific inhibitor of PDE4, inhibited HOSM-1 cell proliferation. The finding that PDE4 is involved in proliferation of osteosarcoma cells suggests the possibility that PDE4 may be a new target for antitumor therapy.Keywords
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