Specific double diffusion microtechnique for the diagnosis of aspergillosis and paracoccidioidomycosis using monospecific antisera

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Abstract
Using experimental reference sera against species-specific antigens of Aspergillus fumigatus and Paracoccidioides brasiliensis in a microdouble diffusion technique, a simple and specific test for immunodiagnosis of aspergillosis and paracoccidioidomycosis was developed. Only sera that produced lines of identity with either one of the bands formed by the anti-C2 or anti-E2 reference sera were considered positive for aspergillosis or paracoccidioidomycosis, respectively. Sensitivity of the diagnostic test was similar to those of the classical double diffusion and immunoelectrophoresis tests. No false positives were found in sera obtained from patients affected by other mycoses, nor from healthy controls. The amount of reagents for the specific test was 10-fold less than that required by the classical double diffusion test.