Incidence of Neutrophil Antigens on Human Cord Neutrophils

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Abstract
Neutrophils isolated from cord blood of healthy newborns (33 blacks and 21 whites) were investigated by EDTA-microagglutination for their expression of neutrophil specific antigens that have been associated with isoimmune neonatal or autoimmune neutropenia. Equal volumes of various neutrophil antisera (2 .mu.l) and cord neutrophils (3-5 .times. 106/ml) were mixed in tissue typing microplates under oil and were incubated at room temperature for 6-8 h, following which the degree of agglutination was noted. All the currently recognized neutrophil antigens are readily demonstrable by antineutrophil antibodies in cord blood, (NA1, 52-54%; NA2, 81-85%; NB1, 95-96%; NC1, 90%; 9A, 29-30%) suggesting that neutrophil antigens are fully expressed at birth.