An FKBP destabilization domain modulates protein levels in Plasmodium falciparum
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Open Access
- 11 November 2007
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 4 (12) , 1007-1009
- https://doi.org/10.1038/nmeth1132
Abstract
To enhance the repertoire of molecular tools for studying malaria parasite biology, we adapted a ligand-regulatable FKBP protein destabilization domain (ddFKBP) for use in P. falciparum. We destabilized the reporter yellow fluorescent protein (YFP) and the P. falciparum protease falcipain-2 in a ligand-reversible manner by tagging with ddFKBP. The swollen food vacuole phenotype of falcipain-2 knockout parasites could be rescued in a Shld1 ligand-dependent fashion by falcipain-2-ddFKBP expression.Keywords
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