Rapid control of protein level in the apicomplexan Toxoplasma gondii
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- 11 November 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (12) , 1003-1005
- https://doi.org/10.1038/nmeth1134
Abstract
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.Keywords
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