Cellular interactions in the generation of cytolytic T lymphocyte responses: role of Ia-positive splenic adherent cells in presentation in H-2 antigen.

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Abstract
Splenic adherent cells are required for generation of primary and, at limiting antigen dose, secondary allogeneic responses of [mouse] cytolytic T lymphocytes to intact stimulator cells. Secondary responses to purified allogeneic plasma membranes or purified H-2Kk antigens in liposomes are dependent upon splenic adherent cells. Generation of these responses requires the presence of an Ia-positive, Thy 1.2-negative, radiation-resistant cell in the splenic adherent cell population that is adherent to glass, plastic and nylon wool. Stimulation of cytolytic T lymphocyte precursors with purified H-2Kk alloantigen bound to Ia+ splenic adherent cells is 10-20 times more efficient than stimulation with antigen added directly to culture. A marked decrease in the response of cytolytic T lymphocytes to liposomes was observed when antiserum against Iad specific for the Ia of the responder cells was added to culture. For purified proteins of the major histocompatibility complex, antigen presentation by Ia+ splenic adherent cells apparently plays a role in the generation of a cytolytic T lymphocyte response.