Abstract
Horse heart cytochrome c with either histidine or cysteine replacing the endogenous axial methionine ligand at position 80 has been characterized with magnetic circular dichroism (MCD) spectroscopy in the UV‐visible region. Comparison of the MCD spectra of the mutant proteins in the ferric state to those of authentic bis‐imidazole‐ and imidazole/thiolate‐ligated ferric heme proteins clearly shows that the histidine‐imidazole and cysteine‐thiolate groups of the replacement amino acids at position 80 are coordinated to the heme iron in the mutant proteins. This study demonstrates the power of MCD spectroscopy in identifying axial ligands in mutant heme proteins. Accurate axial ligand assignment is essential for proper interpretation of the altered properties of such novel proteins.