Electron microscopy of CRFK cells infected with canine parvovirus.

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Abstract
Electron microscopic observation was carried out on the CRFK cells inoculated with canine parvovirus. The cells were synchronized by thymidine before inoculation. The earliest findings were chromatin margination and nucleolar deformation. Virus particles about 20 nm in diameter were first seen, forming clumps in the nucleus 18 hr postinoculation (p.i.). Numerous virus particles were observed in the nucleus 48 hr p. i. They usually formed large aggregates, and were occasionally scattered within the nucleus. The host cells showed marked nuclear deformation and cytoplasmic vacuolation. Virus particles were also found in the degenerated cytoplasmic matrix 48 and 72 hr p.i., forming aggregates, sometimes being associated with membranous structures. These findings suggested that the virions were composed within the nucleus of CRFK cells, and then moved into the cytoplasm.