Euglena gracilischloroplast DNA: the untranslated leader oftufA-ORF206 gene contains an intron
Open Access
- 12 October 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (19) , 7809-7822
- https://doi.org/10.1093/nar/15.19.7809
Abstract
Structural features of a dicistronic 1.95 kb mRNA coding for the chloroplast specific elongation factor Tu and ORF206 are described. The unspliced pre-mRNA is composed of 2562 nucleotides and undergoes four splicing events which remove a total of 606 nucleotides. The first intron splits the untranslated leader, two introns dissect the tufA coding region and the forth intron is within ORF206, which codes for a putative protein that is to 34% homologous with the putative protein of chloroplast ORF184 of tobacco. Introns neither belong to group I nor II, and 5' and 3' intron boundaries do not follow consensus sequences. Potential ribosome binding sites are located 50 and 32 positions upstream of the tufA and ORF206 start codon, respectively.Keywords
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