Reaction of Ethanol Radicals with Ribonuclease
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 30 (5) , 467-472
- https://doi.org/10.1080/09553007614551281
Abstract
Ribonuclease was irradiated under conditions such that ethanol radicals were the main reactive species in solution. Sephadex gel filtration of the irradiated solution demonstrated that ethanol radicals had reacted with the ribonuclease and had become firmly bound to the enzyme molecule. The number of ethanol molecules bound to ribonuclease was a function of dose and correlated with the loss of enzymatic activity and with the changes in the molecular configuration of the enzyme molecule.This publication has 5 references indexed in Scilit:
- Reduction of Ferricytochrome c by Some Free Radical AgentsScience, 1974
- The Effect of Aqueous Alcohol Solutions on the Thermal Transition of Ribonuclease1a,bThe Journal of Physical Chemistry, 1965
- Spectrophotometric assay of bovine pancreatic ribonuclease by the use of cytidine 2′:3′-phosphateBiochemical Journal, 1960