Further evidence for the interconversion of monophosphoinositides in vivo

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Abstract
The incorporation of3H‐inositol into the molecular species of rat liver monophosphoinositides was studied as a function of time. At early time intervals following intraperitoneal injection of the tracer, the specific activity of the monoenoic and dienoic species exceeded that of the total and tetraenoic inoisitides by 7‐ and 14‐fold. From 90 min to 9 hr, the specific activity of the unfractionated phosphatide remained nearly constant while the pronounced decrease in activity [of the monoenoic and dienoic subfractions was concomitant with an increase in the radioactivity of the tetraenoic species, as found previously with32P‐ and14C‐glycerol. The present results suggest that the entire glycerophosphorylinositol backbone originally associated with the monoenoic and dienoic monophosphoinositides is converted into tetraenoic species with remarkable conservation of label in vivo.