Quantitative analysis of phospholipids by thin-layer chromatography

Publisher Website
Google Scholar
Abstract
A procedure has been described for the quantitative separation of phospholipids by thin-layer chromatography. When standard compounds were applied singly and as mixtures to silica-gel plates, average recoveries were within the range 97.9-102.0%. A well-defined separation of phospholipids of rat liver was achieved on silica-gel plates, with an average recovery of 100.8% of the total phosphorus applied. Analyses of lipids from pooled rat livers gave the following results phosphatidylcholine, 55.0%; phosphatidyl-ethanolamine, 25.3%; phosphatidylinositol, 8.8%; phosphatidylserine, 3.0%; sphingomyelin, 1.8%; lysophosphatidylcholine, 0.9%; phosphatidic acid and cardiolipin, 5.1%. This procedure, which permits the determination of the main known phospholipids, is simple, rapid, reproducible and reliable.