Enzymic racemization of optically active lactic acid

Abstract
Formation of inactive lactic acid in associated growth of Strep. lactis or Lactobacillus delbruckii with Clostridium aceto-butylicum or Cl. butylicum was caused by the racemizing action of the butyric acid organisms on the active acid produced by these lactic acid bacteria. Cl. acetobutylicum and Cl. butylicum produced enzyme systems which changed either d- or l-lactic acid quantitatively into dl-acid in the presence of antiseptics. The racemizing system seemed to consist of 2 components, separated by centrifuging a culture of the organisms; a heat stable intracellular fraction, and a heat labile (87[degree]C, 10 min.) extracellular fraction.

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