CHEMOTHERAPY SENSITIVITY ASSESSMENT OF LEUKEMIC COLONY-FORMING CELLS WITH INVITRO SIMULTANEOUS EXPOSURE TO MULTIPLE-DRUGS - CLINICAL CORRELATIONS IN ACUTE NONLYMPHOCYTIC LEUKEMIA
- 1 January 1982
- journal article
- research article
- Vol. 66 (6) , 1257-1261
Abstract
For 13 patients with acute nonlymphocytic leukemia receiving 4-drug combination chemotherapy [vincristine, cytarabine and prednisone with doxorubicin or zorubicin] (14 chemotherapy trials), leukemic bone marrow cells were obtained before treatment and were exposed in vitro to all 4-drug-mixture solutions simultaneously followed by the survival assessment of leukemic colony-forming cells (L-CFU). Survival of normal marrow colony-forming cells (CFU-C [granulocytic progenitor cells]) was also assessed concurrently; the sensitivity index (SI) of leukemic cells was determined as the ratio of the survival of CFU-C to that of L-CFU. Correlations of in vitro results to clinical results were excellent. Four of 5 trials resulting in complete remission had high SI; 8 of 9 trials without complete remission had low SI (P = 0.02). The potential advantages of this method of exposing cells to drug mixture are discussed in terms of detecting drug synergism and improving the efficiency of in vitro chemotherapy sensitivity studies.This publication has 4 references indexed in Scilit:
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