Chemical characterization of the regularly arranged surface layer glycoprotein of Clostridium thermosaccharolyticum D120‐70
Open Access
- 1 February 1990
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 188 (1) , 73-82
- https://doi.org/10.1111/j.1432-1033.1990.tb15373.x
Abstract
Clostridium thermosaccharolyticum D120-70 possesses as its outermost cell envelope layer a square-arranged array of glycoprotein molecules. SDS/polyacrylamide gel electrophoresis of the purified surface layer showed a broadened band in the molecular mass range of about 115 kDa which, upon periodic acid/Schiff staining, gave a positive reaction. After proteolytic degradation of this material, two glycopeptide fractions were obtained. One-and two-dimensional nuclear magnetic resonance studies, together with methylation analysis and periodate oxidation, were used to determine the structures of the polysaccharide portions of these glycopeptides. The combined chemical and spectroscopic evidence suggests the following structures:This publication has 36 references indexed in Scilit:
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