INFLUENCE OF ADRIAMYCIN AND ADRIAMYCIN-RADIATION COMBINATION ON JEJUNAL PROLIFERATION IN MOUSE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (1) , 22-27
Abstract
The influence of the antitumor agent adriamycin and adriamycin-radiation combinations of posttreatment proliferative activity of the mouse jejunum was examined by measuring [3H]thymidine incorporation. Single doses of 5 or 10 mg/kg produced a transient reduction in proliferative activity, while 1 mg/kg had little effect. After 10 mg/kg, there was a rapid decrease in the number of mitotic figures, followed by a gradual decrease in the number of and rate of DNA synthesis in S-phase cells. A compensatory epithelial hyperplasia characterized by an enlarged crypt proliferative population and shortened mitotic cycle duration was observed beginning 48 h after treatment. Multiple doses of adriamycin totaling 10 mg/kg inhibited cell production to a greater extent than the equivalent single dose. In combination with 1000 R, adriamycin (5 mg/kg) given from 96 h before to 72 h after irradiation reduced the amount of postirradiation proliferation.