Studies on peptides. CXXXVI. Solution-phase synthesis of a 37-residue peptide amide corresponding to the entire amino acid sequence of human calcitonin gene-related peptide (hCGRP).
- 1 January 1986
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (2) , 613-620
- https://doi.org/10.1248/cpb.34.613
Abstract
The heptatriacontapeptide amide corresponding to the entire amino acid sequence of human calcitonin gene-related peptide (hCGRP) was synthesized by assembling seven peptide fragments in solution, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid and subsequent air-oxidation to establish the disulfide bridge. The S-1-adamantyl group of the Cys residue was removed in two ways; one was by treatment with the above acid, together with the other protecting groups employed, and the other was selectively by treatment with(CF3COO)3Tl. The synthetic peptide produced a significant increase of cyclic adenosine monophosphate in calvaria and brain of a newborn rat, but suppressed 45Ca-release from mouse calvaria stimulated by parathyroid hormone.Keywords
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