A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin
Open Access
- 1 July 1992
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 15 (1) , 23-25
- https://doi.org/10.1111/j.1472-765x.1992.tb00714.x
Abstract
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.Keywords
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