Quantitation of Lactate Dehydrogenase of Developing Molar Teeth of the Mouse

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Abstract
Homogenates of developing 1st molar teeth of 2-21 day old mice were analyzed by a quantitative spectrophotometric technique and acrylamide gel electrophoresis for lactate dehydrogenase activity. Tissue sections of these teeth were prepared and the enamel and dentin widths measured. The protein content of the teeth was determined and the wet weights recorded. The enamel and dentin widths, the wet weight and the protein content all increased with increasing tooth age, expressing the stages of mouse molar development. Lactate dehydrogenase (LDH) activity measured in m[mu] moles NADH formed/min./mg wet weight of tissue and in m[mu] moles NADH formed/ min./[mu]g protein decreased with increasing tooth age. Five lactate dehydrogenase isozymes from the teeth were demonstrated after acrylamide gel electrophoresis. There was a decrease in the activity of all the isozymes with increasing age of the tooth. The 2 fastest moving isozymes could not be demonstrated after 10 days. The LDH isozymes were identical in electrophoretic mobility to the isozymes from mouse heart muscle, liver, kidney, skeletal muscle and serum. An exact role and mechanism of LDH in the developing tooth can not be defined.