Expression of circumsporozoite proteins revealedin situin the mosquito stages ofPlasmodium bergheiby the Lowicryl-immunogold technique

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Abstract
A monoclonal antibody against the circumsporozoite proteins of the Plasmodium berghei sporozoite was used to trace the synthesis and expression of these proteins, via the Lowicryl immunogold technique, within the developing oocyst. The proteins were detected on the endoplasmic reticulum of the oocyst and were present in the sporozoite membranes at the point of their formation.