Increased Collagen Deposition and Elevated Expression of Connective Tissue Growth Factor in Human Thoracic Aortic Dissection
- 4 July 2006
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation
- Vol. 114 (1_suppleme) , I200-5
- https://doi.org/10.1161/circulationaha.105.000240
Abstract
Background— Thoracic aortic dissection (TAD) is characterized by dysregulated extracellular matrix. Little is known about the alterations of collagen and stimulators of collagen synthesis, eg, connective tissue growth factor (CTGF), in patients with TAD. In this study, we examined their roles in TAD. Methods and Results— Surgical specimens of the aortic wall of TAD patients (n=10) and controls (n=10) were tested for collagen types I and III and CTGF expression. When compared with controls, protein levels of type I and III collagen and CTGF were significantly increased by 3.2-, 3.7-, and 5.3-fold, respectively (P<0.05 for all). Similar patterns were shown in mRNA levels of type Iα and Iα2 collagen and CTGF. Using immunohistochemistry and trichrome staining, we also observed elevated levels of collagen in the aortic media and adventitia. Treatment with recombinant human CTGF increased collagen synthesis in cultured aortic smooth muscle cells in a dose- and time-dependent fashion, in which expression of coll...Keywords
This publication has 24 references indexed in Scilit:
- Connective Tissue Growth Factor Is Overexpressed in Complicated Atherosclerotic Plaques and Induces Mononuclear Cell Chemotaxis In VitroArteriosclerosis, Thrombosis, and Vascular Biology, 2005
- Combinatorial signaling pathways determine fibroblast proliferation and myofibroblast differentiationThe FASEB Journal, 2004
- Mice With a Deletion in the First Intron of the Col1a1 Gene Develop Age-Dependent Aortic Dissection and RuptureCirculation Research, 2004
- Abnormal Extracellular Matrix Protein Transport Associated With Increased Apoptosis of Vascular Smooth Muscle Cells in Marfan Syndrome and Bicuspid Aortic Valve Thoracic Aortic AneurysmCirculation, 2003
- Connective Tissue Growth Factor: What's in a Name?Molecular Genetics and Metabolism, 2000
- Regulation of Tenascin-C, a Vascular Smooth Muscle Cell Survival Factor that Interacts with the αvβ3 Integrin to Promote Epidermal Growth Factor Receptor Phosphorylation and GrowthThe Journal of cell biology, 1997
- Purification and Characterization of Novel Heparin-binding Growth Factors in Uterine Secretory FluidsPublished by Elsevier ,1997
- Collagen: scaffold for repair or executionCardiovascular Research, 1997
- Collagen types and matrix protein content in human abdominal aortic aneurysmsJournal of Vascular Surgery, 1989
- Transmural organization of the arterial media. The lamellar unit revisited.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1985