Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates
Open Access
- 1 September 2007
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 56 (9) , 1181-1184
- https://doi.org/10.1099/jmm.0.47346-0
Abstract
A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8 %) of 153 isolates; 126 (82.4 %) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.Keywords
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