Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children
- 1 September 2007
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 56 (9) , 1185-1188
- https://doi.org/10.1099/jmm.0.47347-0
Abstract
Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.Keywords
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