Immobilisierung partikelgebundener Aldehyddehydrogenase aus Acinetobacter calcoaceticus EB 104
- 12 January 1985
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 25 (7) , 429-435
- https://doi.org/10.1002/jobm.3620250705
Abstract
Particle‐bound aldehyde dehydrogenase from Acinetobacter calcoaceticus was attached to octylsepharose by hydrophobic membrane proteins without previous solubilization. To optimize this method, the influence of the pH‐value of immobilization, ammonium sulfate concentration, presence of tensides, and some other substances on relative activity and yield was investigated. Best results were obtained when immobilization was carried out for 1–2 h at 4°C in 2moles/l KCl, pH 7.5. The optimum pH for storage was 9.0 and the maximum half‐life 40 days at 4°C or 18 days at 25°C.This publication has 17 references indexed in Scilit:
- Isolierung und Charakterisierung der Zellgrenzschichten vonAcinetobacter calcoaceticusJournal of Basic Microbiology, 1977
- Induktion der NADP+-abhängigen Aldehyddehydrogenase durch Kohlenwasserstoffe beiAcinetobacter calcoaceticusJournal of Basic Microbiology, 1977
- Isolierung und Charakterisierung der Zellgrenzschichten von Acinetobacter calcoaceticusJournal of Basic Microbiology, 1977
- Induktion der NADP+‐abhängigen Aldehyddehydrogenase durch Kohlenwasserstoffe bei Acinetobacter calcoaceticusJournal of Basic Microbiology, 1977
- Affinity chromatography of enzyme cofactors: The separation of NAD on immobilized dehydrogenase columnsBiochimica et Biophysica Acta (BBA) - Enzymology, 1975
- Aldéhyde déshydrogénases soluble et particulaire de Pseudomonas aeruginosaBiochimie, 1973
- Isolation and properties of succinate dehydrogenase from Rhodospirillum rubrumArchives of Biochemistry and Biophysics, 1972
- Succinate dehydrogenase. I. Purification, molecular properties, and substructureBiochemistry, 1971
- Batch purification of ovomucoid and characterization of the purified productBiochemistry, 1971
- Entrapment of Enzymes and Microorganisms in Synthetic Cross-linked Polymers and their Application in Column Techniques.Acta Chemica Scandinavica, 1966