The Isolation of a Bacteriolytic Principle from the Root Nodules of Red Clover

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Abstract
A filter-passing, heat-labile lytic principle, specific for the organism causing the root nodule from which the principle was isolated, was passed through 20 serial transfers, with neither decrease nor marked increase in activity. Bacteria isolated from secondary growth which developed on incubation of a lysed culture resisted the action of the principle, but still produced nodules on red clover; continued growth within the nodule apparently resulted in loss of resistance, whereas after 8 months'' cultivation on artificial media the resistance persisted. Attempts to produce lysis on solid media failed.

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