Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
Open Access
- 7 September 2010
- journal article
- Published by Springer Nature in Microbial Cell Factories
- Vol. 9 (1) , 65
- https://doi.org/10.1186/1475-2859-9-65
Abstract
The increasing regulatory requirements to which biological agents are subjected will have a great impact in the field of industrial protein expression and production. There is an expectation that in a near future, there may be "zero tolerance" towards antibiotic-based selection and production systems. Besides the antibiotic itself, the antibiotic resistance gene is an important consideration. The complete absence of antibiotic-resistance gene being the only way to ensure that there is no propagation in the environment or transfer of resistance to pathogenic strains.Keywords
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