Immunocytochemical localization of sphingolipid activator protein-1, the sulfatide/GM1 ganglioside activator, to lysosomes in human liver and colon
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 86 (2) , 195-200
- https://doi.org/10.1007/bf00493387
Abstract
Sphingolipid activator proteins (SAP) stimulate the enzymatic hydrolysis of sphingolipids. The results of biochemical studies have suggested that SAP are located within lysosomes. In this study we sought immunocytochemical verification of the lysosomal location of SAP-1, a SAP that stimulates the hydrolysis of sulfatide and GM1 ganglioside. We stained adjacent sections of normal adult liver and colon for either SAP-1, by peroxidase-labeled antibodies, or acid phosphatase, by enzyme histochemistry. At the light microscopic level, SAP-1 and acid phosphatase were present in similar cells of the colonic lamina propria and hepatic sinusoids, and in similar supranuclear sites of colonic epithelial cells. By electron microscopy, SAP-1 was present in vesicular structures morphologically similar to those containing acid phosphatase. Thus, SAP-1 is present in lysosomes of several different kinds of cells in the normal human liver and colon.This publication has 19 references indexed in Scilit:
- Biosynthesis of the sulfatide/GM1 activator protein (SAP-1) in control and mutant cultured skin fibroblastsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Molecular cloning of the sphingolipid activator protein — 1 (SAP - 1), the sulfatide sulfatase activatorBiochemical and Biophysical Research Communications, 1986
- Concentrations of an activator protein for sphingolipid hydrolysis in liver and brain samples from patients with lysosomal storage diseases.Journal of Clinical Investigation, 1983
- A protein activator of galactosylceramide β-galactosidaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- A variant form of metachromatic leukodystrophy without arylsulfatase deficiencyAnnals of Neurology, 1982
- STUDIES ON TRANSLOCATION OF IMMUNOGLOBULINS ACROSS INTESTINAL EPITHELIUMACTA HISTOCHEMICA ET CYTOCHEMICA, 1976
- PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATIONJournal of Histochemistry & Cytochemistry, 1974
- PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPYJournal of Histochemistry & Cytochemistry, 1974
- Eine Cerebrosidsulfatase aus SchweineniereHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1964
- Lysosomes in the Physiology and Pathology of Cells: Contributions of Staining MethodsPublished by Wiley ,1963