Isolation of the chicken thymidine kinase gene by plasmid rescue
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 285 (5762) , 207-210
- https://doi.org/10.1038/285207a0
Abstract
The bacterial plasmid pBR322 was used as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. The chicken thymidine kinase (tk) gene was obtained as a 2.2-kilobase EcoRI/HindIII insert in pBR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.Keywords
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