CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS

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Abstract
From 90 to 130 mg (dry weight) of spores (about 1% dark forms) were obtained (per liter) from a chemically defined, synthetic medium, with a two-phase (polyethylene-glycol-potassium-phosphate) harvest procedure. Optimal sporulation occurred when glucose and glutamic-acid were at a concentration of 10 m[image] in the medium. Ca++ and Mn++ were required for sporulation. Heat resistance, dipico-linic-acid content and properties of germination and postgerminative development of spores grown in different concentrations of C++ were investigated. Heat shock did not increase germination of spores derived from the synthetic medium. A synthetic medium, in which spore germination, emergence and first cell division approached synchrony was devised.