Effect of 2-Deoxyglucose on Schizosaccharomyces pombe

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Abstract
Cultivation of Schizosaccaromyces pombe in a medium containing 2-deoxyglucose (100[mu]g/ml) results in the death of the cells after an initial period of apparently normal growth. At higher deoxyglucose concentration (400[mu]g/ml), the cells die immediately after inoculation. Only growing cells are killed, and microscopic inspection of the cultures reveals cell-wall fragments of lysed cells. A mutant resistant to 2-deoxyglucose, which cannot use glucose as a carbon source, was found to be partially deficient in hexokinase. The data constitute evidence for the inhibition of some reaction(s) in the synthesis of cell-wall poly-saccharides by metabolites of 2-deoxyglucose in this organism.