Antibody-forming Cell Assays of Avian Paramyxoviruses: the Serotype-specific Response of Mice
- 1 February 1989
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 70 (2) , 315-323
- https://doi.org/10.1099/0022-1317-70-2-315
Abstract
Mice were immunized with eight different serotypes of avian paramyxovirus (PMV). The number of splenocytes that formed antibody to PMV-infected Madin-Darby bovine kidney cells was determined by immunoperoxidase staining. Microscopic examination revealed brown plaques of cell surface or inclusion body-like antigen comprising the glycoprotein or nucleoprotein-polymerase complex, respectively. The IgG response to virus glycoprotein was priming-dependent and 99% serotype-sepcific, a value which exceeds that of PMV typing using avian serology. The response to inclusion body-like antigens was more variable and less priming-dependent and suggested cross-reactivities betwen PMV-1 and PMV-3 or -9. The primary IgM response also included a 1 to 10% non-specific antibody responses to host antigens revealed by neuraminidase treatment.This publication has 14 references indexed in Scilit:
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