CYTOTOXIC TYPING ANTISERA FOR MARROW GRAFTING IN LITTERMATE DOGS

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Abstract
SUMMARY Immunization of dogs with buffy coat cells from unrelated dogs consistently produced cytotoxic antisera which gave strong reactions but were too complex for suitable analysis. Cross immunization of littermate pairs produced cytotoxic antisera only infrequently, and these antisera gave weaker reactions. Four antisera were obtained by sibling immunization. They showed limited specificity as determined by cross absorption studies and typing of canine families. Genetic studies indicated that these antisera recognized independent antigens of dominant inheritance. Testing of lymphocytes from 62 unrelated dogs with these four antisera gave positive reactions with frequencies of 0.55, 0.53, 0.31, and 0.26. In order to evaluate the usefulness of these sera for histocompatibility typing, six litters were typed with the four antisera. A donor was selected from each litter, and an aliquot of marrow was infused into a matched and into a mismatched sibling following 1500r of whole-body irradiation. None of the mismatched recipients lived longer than 14 days. All of the matched recipients lived beyond 40 days, and 4 of the 6 lived more than 100 days. It was concluded that cross immunization of litter mates produced reasonably specific typing sera. These sera were of value in selecting donor-recipient pairs of canine siblings for marrow grafting.